Since 1985, when the first protein il2 was produced in large scale from a recombinant baculovirus. It is a nuclear polyhedrosis virus npv of the family. The israeli journal of aquaculture bamidgeh 6, 2009 fig. Penaeus monodon baculovirus variously known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv or monodon baculovirus mbv, the causative agent of spherical baculovirosis in shrimp p. Two years later, it was designated monodon baculovirus mbv 2, and this name is still commonly used today. World production of shrimp from capture fisheries and aquaculture. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported. Prevalence of monodon baculovirus mbv in wild shrimp penaeus. Baculovirus infections may result in disease in cultured penaeids that is accompanied by high mortality rates. Ancient coevolution of baculoviruses and their insect hosts. In the wild, they show marked nocturnal activity, burrowing into bottom substratum during the day and emerging at night to search for food as benthic feeders. The genome and occlusion bodies of marine penaeus monodon. Major viral diseases of penaeus monodon in taiwan chufang lo, yunshiang chang, shoen peng and guanghsiung kou received, march 10, 2003.
The present study attempts to investigate the effect of penaeus monodon. Several investigators have demonstrated that prawn primary cell cultures can support in vitro growth of monodon baculovirus. Manual of histological staining methods of the armed forces. Multiple viral pathogens occurrence in tiger shrimp.
The trees were found by a heuristic search starting with neighbor joining and nearestneighbor. White spot baculovirus syndrome in the indian shrimp penaeus monodon and p. Focusing on the giant tiger shrimp penaeus monodon. Decontamination of monodon baculovirus in marine shrimp eggs using. However, hznv2 lacks several key genetic features of baculoviruses including the late transcriptional regulation factor, lef1. A survey on the presence of the viruses of two economically significant diseases, white spot syndrome virus wssv and monodon baculovirus mbv in wild. Penaeus monodon is the secondmost widely cultured prawn species in the world, after only whiteleg shrimp, litopenaeus vannamei. Guide to baculovirus expression vector systems bevs and. Production was dominated by penaeus monodonuntil 2002 and by penaeus vannamei thereafter. Another less accurate process, microhomologymediated end joining is also used to. Even though mbv is not a severe pathogen for the black tiger prawn penaeus monodon, it should be eliminated from the farming system because it is unlikely that.
The objective of our study was to explain the histopathological changes of monodon baculovirus mbv in hepatopancreas and midgut tissues of the cultured penaeus vannamei. Us6143547a cell line derived from penaeus monodon and a. Occurrence of viral pathogens in penaeus monodon post. Monodon baculovirus mbv mbv is the first reported virus of p. Decontamination, monodon baculovirus, mbv, shrimp egg, upwelling flowthrough system. The present invention relates to the establishment of an immortal shrimp cell line pmo derived from penaeus monodon. A record and prevalence of monodon baculovirus from. Monodon baculovirus mbv forms polyhedral occlusion bodies obs. Detection of monodon baculovirus and whitespot syndrome. Penaeus monodon type baculovirus tetrahedral baculovirosis baculovirus penaei taura syndrome white spot disease white tail disease yellowhead disease. Penaeus monodon mature and breed only in tropical marine habitats and spend their larval, juvenile, adolescent and subadult stages in coastal estuaries, lagoons or mangrove areas. Cell membranes can also blend or merge without fusion of the cellular contents.
Poor quality seed, coupled with the slow growth of p. Shrimp, penaeus semisulcatus, penaeus monodon baculovirus, histopathology. Penaeus monodon, commonly known as the giant tiger prawn or asian tiger shrimp is a marine. Millions of metric tons dominated by penaeus monodon. Analysis of the genome of the sexually transmitted insect. Evidence for the presence of white spot syndrome virus. The pcr was shown to be specific for mbv dna by not amplifying prawn dna or dna preparations of baculovirus penaei bp, white spot baculovirus wsbv, bennettae baculovirus and insect. Penaeus monodontype baculovirus mbv is found in many parts of the world from. Environmental assessment report import of specific. Monodon baculovirus mbv is an economically important and one of the. The simultaneous presence of monodon baculovirus mbv and whitespot syndrome virus wssv in apparently healthy postlarvae of penaeus monodon from different hatcheries in india was studied by. Monodon baculovirus mbv exists in populations of the indian tiger prawn, penaeus monodon, obtained from a commercial hatchery in madras, india.
Diseases such as whitespot baculovirus and yellowhead virus have led to a great. Polymerase chain reaction pcr assays have been reported for specific and accurate. Request pdf prevalence of monodon baculovirus mbv in wild shrimp. Ultrastructural and pathogenesis of monodon baculovirus in.
Monodon baculovirus mbv is a member of nucleopolyhedrosis virus npv group that infect penaeid shrimps. Decontamination of monodon baculovirus in marine shrimp eggs using upwelling flowthrough system article pdf available july 2015 with 110 reads how we measure reads. Pdf penaeus monodon type baculovirus mbv is a horizontal transmission virus disease especially found in marine shrimp. In hatcheries, bp and bmn often cause serious epizootics in the larval and early. Two years later, it was designated monodon baculovirus mbv 2. Pdf prevalence of white spot syndrome virus and monodon. Domestication of black tiger shrimp penaeus monodon in recirculation. Wssv, yellow head virus yhv, monodon baculovirus mbv and to a lesser extent, luminescent bacterial infections. Necrosois virus, monodon baculovirus, white spot syndrome virus.
Penaeus monodon baculovirus mbv is a rodshaped virus possessing a. Prevalence of white spot syndrome virus and monodon baculovirus in penaeus monodon broodstock and postlarvae from hatcheries in southeast coast of india. Adw doesnt cover all species in the world, nor does it include all the latest scientific. Occurrence of viral pathogens in penaeus monodon postlarvae.
Pdf prevalence of white spot syndrome virus wssv and. Freepenaeus monodon hatchery manual based on the experience in india. The cytopathology, virogenesis and replication of monodon baculovirus mbv in penaeus monodon from australia are described. Colourimetric pcrbased detection of monodon baculovirus.
Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely. Occurrence of viral pathogens in penaeus monodon postlarvae from aquaculture hatcheries toms c. Prevalence of white spot syndrome virus wssv and monodon baculovirus mbv infection in penaeus monodon postlarvae in vietnam. Current status of viral diseases in asian shrimp aquaculture. The multiplex pcr master mix comprised of 2 l of 10x primer mix.
This cell line is susceptible to and capable of mass producing virus, which includes, but not limited to, white spot syndrome virus wssv, panaeus monodon baculovirus. More than 600 recombinant genes have been expressed in baculoviruses to date. The pcr reactions were done as described in oie manual of diagnosis for aquatic animals 28. White spot baculovirus syndrome in the indian shrimp.
The effect of viral infection on the relationship between. Mbv virions are rodshaped, nonoccluded or occluded. The open access israeli journal of aquaculture bamidgeh. Monodon baculovirus mbv disease viral infections in penaeid shrimps affected shrimps exhibit palebluishgray to dark blueblack coloration, sluggish and inactive swimming movements, loss of. Onfarm feed management practices for black tiger shrimp. Mbv virions are rodshaped, nonoccluded or occluded and enveloped within a paracrystalline occlusion body. We report the use of recombinant baculovirus mediated transduction using hybrid promoter system for transgene expression in lymphoid cells of penaeus monodon. Monodon baculovirus mbv disease viral infections in. Among the viruses infecting penaeid shrimp, monodon type baculovirus mbv otherwise known as penaeus monodon singly enveloped nuclear polyhedrosis virus pmsnpv, is one of the widely reported and well described viruses. Another less accurate process, microhomology mediated end joining is also used to. Spherical baculovirosis penaeus monodon type baculovirus p. Monodon baculovirus has been known to be invasive in 85100% of the shrimp hatcheries, in early or late stages of shrimp. Attempts at producing a hybridised penaeus mondon cell line by cellular fusion.
Mbv monodon baculovirus the occlusion bodies ob formed as monodon baculovirus mbv replicate are easily visible using a microscope. There appear to be more ob when pl are stressed, so. Decontamination of monodon baculovirus in marine shrimp. Improvement of pcr method for the detection of monodon. Pdf decontamination of monodon baculovirus in marine. Monodon baculovirus mbv infects epithelia1 cells of the hepatopancreas hp and midgut of penaeus monodon and other penaeid shrimp. It is a rodshaped, enveloped, doublestranded dna virus, and considered till recently, as the type a baculovirus. Attempts at producing a hybridised penaeus mondon cell.
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